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            Free, publicly-accessible full text available January 1, 2026
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            Abstract Drylands are highly vulnerable to climate change due to their fragile ecosystems and limited ability to adapt. In contrast to the global drying after tropical volcanic eruptions shown previously, we demonstrate that large tropical volcanic eruptions can induce significant two-year hydroclimatic wetting over drylands by employing the last millennium simulations. During this wetting period, which extends from the first to the third boreal winter after the eruption, several hydroclimatic indicators, such as self-calibrating Palmer Drought Severity Index based on the Penman-Monteith equation for potential evapotranspiration (scPDSIpm), standard precipitation evapotranspiration index (SPEI), aridity index (AI), top-10cm soil moisture (SM10cm), and leaf area index (LAI), show significant positive anomalies over most drylands. The primary contribution to the wetting response is the potential evapotranspiration (PET) reduction resulting from dryland surface cooling and reduced solar radiation, as well as a weak contribution from increased precipitation. The latter is due to the wind convergence into drylands caused by slower tropical cooling compared to drylands. The wetting response of drylands to volcanic eruptions also demonstrates some benefits over the global hydrological slowdown resulting from stratospheric aerosol injection, which replicates the cooling effects of volcanic eruptions to address global warming.more » « lessFree, publicly-accessible full text available December 1, 2025
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            Free, publicly-accessible full text available January 1, 2026
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            Free, publicly-accessible full text available January 1, 2026
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            Chlamydiae represent a diverse group of obligate intracellular bacteria with elusive hosts in environmental settings. This study used one of the largest collections of wild amoebae (Dictyostelium discoideum and D. giganteum, 106 clones) collected over the past two decades to screen for novel environmental chlamydiae. We found that novel environmental chlamydiae are prevalent in two wild Dictyostelium species and assembled 42 novel chlamydiae metagenome-assembled genomes (MAGs). The MAGs represent three chlamydiae species previously only reported using 16S sequencing. Their genomes are divergent enough from other species to warrant placing them in two new genera (tentatively called Ca. Dictychlamydia sp. LF1, Ca. Dictychlamydia sp. LF2, and Ca. Feichlamydia sp. LF3). In addition, these chlamydiae species show strong host specificity with two Dictyostelium amoeba hosts, except one amoeba sample. Ca. Dictychlamydia sp. LF1 and Ca. Feichlamydia sp. LF3 was exclusively observed in D. discoideum, while Ca. Dictychlamydia sp. LF2 was found only in D. giganteum. Phylogenetic and comparative genomic analyses suggest that all three chlamydiae are close to arthropod-associated chlamydiae and likely have some intermediate characteristics between previously reported amoeba-associated and vertebrate-associated chlamydiae. This study significantly broadens our understanding of the chlamydial host range and underscores the role of amoebae as vital hosts for environmental chlamydiae.more » « lessFree, publicly-accessible full text available November 1, 2025
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